Glucose-limited fed-batch culture of Escherichia coli for production of recombinant human interleukin-2 with the DO-stat method

Abstract

Fed-batch culture employing the DO-stat method was carried out to produce recombinant human interleukin-2 in Escherichia coli. The concentration of interleukin-2 and final cell density in the fed-batch culture were 1.02 g/l and 60 OD (optical density), respectively. This represents a significant improvement in interleukin-2 production and final cell density over the equivalent values of batch fermentation.