Biodegradation potential of polyethylene terephthalate by the two insect gut symbionts Xanthomonas sp. HY-74 and Bacillus sp. HY-75

Abstract

Polyethylene terephthalate (PET) is a plastic material that is widely used in beverage bottles, food packaging, and other consumer products, which is highly resistant to biodegradation. In this study, we investigated the effects of two insect gut symbionts, Xanthomonas sp. HY-74 and Bacillus sp. HY-75, during PET biodegradation. Both strains degraded PET-containing agar plates, and the sole nutrition source assay showed that HY-74 had different degradation rates depending on the presence of specific carbon and nitrogen sources, whereas HY-75 exhibited comparable degradation across all tested conditions. The two strains biodegraded the PET film with 1.57 ± 0.21% and 1.42 ± 0.46% weight loss after 6 weeks, respectively. Changes in the morphology and structure of the PET films, such as erosion, scratching, and surface roughening, were determined using scanning electron microscopy (SEM). Further, the two strains biodegraded PET powder, broke it into its degradation products, and changed the surface functional groups. This is the first study to investigate the biodegradation of PET by Hymenoptera gut-derived microbes and offers promising insights into the potential applications of insect gut symbionts in PET waste management.