Rapid and simple purification of human immunodeficiency virus 1 epitope gp41

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dc.contributor.authorMi Jin Sohn-
dc.contributor.authorSeo Hee Cho-
dc.contributor.authorWon-Hee Jang-
dc.contributor.authorYoung-Hae Chong-
dc.contributor.authorSang Uk Nham-
dc.contributor.authorYoung Ik Lee-
dc.coverage.temporal20180101-20180712-
dc.date.accessioned2017-04-19T08:44:17Z-
dc.date.available2017-04-19T08:44:17Z-
dc.date.issued1993-
dc.identifier.issn0166-0934-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3263-
dc.description.abstractIn order to develop a reliable and inexpensive serodiagnostic method, part of the transmembrane glycoprotein gene of HIV-1, gp41', (HIV-env 548-646) was cloned into an expression vector, pCT10 with a sequence encoding a hydroxylamine cleavage site and with a part of Lac Z gene (Lac 2'': 834 base pairs) as a fusion partner. Overexpression of Lac Z''-gp41' was induced in E. coli and the gp41' fusion protein was purified to homogeneity by centrifugation, hydroxylamine cleavage and an ion-exchange chromatography. Western blot analysis and enzyme-linked immunosorbant assay (ELISA) using the purified gp41 fragment showed high sensitivity and specificity of gp41 as an antigen to detect anti HIV-1 antibodies in testing human sera. These results suggest that this simple and rapid purification method is reliable for obtaining a large quantity of purified gp41'.-
dc.publisherElsevier-
dc.titleRapid and simple purification of human immunodeficiency virus 1 epitope gp41-
dc.title.alternativeRapid and simple purification of human immunodeficiency virus 1 epitope gp41-
dc.typeArticle-
dc.citation.titleJournal of Virological Methods-
dc.citation.number1-
dc.citation.endPage100-
dc.citation.startPage93-
dc.citation.volume41-
dc.contributor.affiliatedAuthorMi Jin Sohn-
dc.contributor.affiliatedAuthorSang Uk Nham-
dc.contributor.affiliatedAuthorYoung Ik Lee-
dc.contributor.alternativeName손미진-
dc.contributor.alternativeName조서희-
dc.contributor.alternativeName장원희-
dc.contributor.alternativeName정영혜-
dc.contributor.alternativeName남상욱-
dc.contributor.alternativeName이영익-
dc.identifier.bibliographicCitationJournal of Virological Methods, vol. 41, no. 1, pp. 93-100-
dc.identifier.doi10.1016/0166-0934(93)90165-N-
dc.subject.keywordgp41-
dc.subject.keywordhuman immunodeficiency virus-1-
dc.subject.keywordpurification-
dc.subject.localgp41-
dc.subject.localgp41'-
dc.subject.localHuman immunodeficency virus-1-
dc.subject.localhuman immunodeficiency virus-1-
dc.subject.localPurifcation-
dc.subject.localPurification-
dc.subject.localpurification-
dc.description.journalClassY-
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