DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mi Jin Sohn | - |
dc.contributor.author | Seo Hee Cho | - |
dc.contributor.author | Won-Hee Jang | - |
dc.contributor.author | Young-Hae Chong | - |
dc.contributor.author | Sang Uk Nham | - |
dc.contributor.author | Young Ik Lee | - |
dc.coverage.temporal | 20180101-20180712 | - |
dc.date.accessioned | 2017-04-19T08:44:17Z | - |
dc.date.available | 2017-04-19T08:44:17Z | - |
dc.date.issued | 1993 | - |
dc.identifier.issn | 0166-0934 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3263 | - |
dc.description.abstract | In order to develop a reliable and inexpensive serodiagnostic method, part of the transmembrane glycoprotein gene of HIV-1, gp41', (HIV-env 548-646) was cloned into an expression vector, pCT10 with a sequence encoding a hydroxylamine cleavage site and with a part of Lac Z gene (Lac 2'': 834 base pairs) as a fusion partner. Overexpression of Lac Z''-gp41' was induced in E. coli and the gp41' fusion protein was purified to homogeneity by centrifugation, hydroxylamine cleavage and an ion-exchange chromatography. Western blot analysis and enzyme-linked immunosorbant assay (ELISA) using the purified gp41 fragment showed high sensitivity and specificity of gp41 as an antigen to detect anti HIV-1 antibodies in testing human sera. These results suggest that this simple and rapid purification method is reliable for obtaining a large quantity of purified gp41'. | - |
dc.publisher | Elsevier | - |
dc.title | Rapid and simple purification of human immunodeficiency virus 1 epitope gp41 | - |
dc.title.alternative | Rapid and simple purification of human immunodeficiency virus 1 epitope gp41 | - |
dc.type | Article | - |
dc.citation.title | Journal of Virological Methods | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 100 | - |
dc.citation.startPage | 93 | - |
dc.citation.volume | 41 | - |
dc.contributor.affiliatedAuthor | Mi Jin Sohn | - |
dc.contributor.affiliatedAuthor | Sang Uk Nham | - |
dc.contributor.affiliatedAuthor | Young Ik Lee | - |
dc.contributor.alternativeName | 손미진 | - |
dc.contributor.alternativeName | 조서희 | - |
dc.contributor.alternativeName | 장원희 | - |
dc.contributor.alternativeName | 정영혜 | - |
dc.contributor.alternativeName | 남상욱 | - |
dc.contributor.alternativeName | 이영익 | - |
dc.identifier.bibliographicCitation | Journal of Virological Methods, vol. 41, no. 1, pp. 93-100 | - |
dc.identifier.doi | 10.1016/0166-0934(93)90165-N | - |
dc.subject.keyword | gp41 | - |
dc.subject.keyword | human immunodeficiency virus-1 | - |
dc.subject.keyword | purification | - |
dc.subject.local | gp41 | - |
dc.subject.local | gp41' | - |
dc.subject.local | Human immunodeficency virus-1 | - |
dc.subject.local | human immunodeficiency virus-1 | - |
dc.subject.local | Purifcation | - |
dc.subject.local | Purification | - |
dc.subject.local | purification | - |
dc.description.journalClass | Y | - |
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