Expression of a chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus in murine myeloma cell line

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Title
Expression of a chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus in murine myeloma cell line
Author(s)
Byung Rae Jin; Chun Jeih Ryu; Sung Sup Park; Hyo Jeong Hong; Moon Hi Han
Bibliographic Citation
Molecules and Cells, vol. 3, no. 1, pp. 7-12
Publication Year
1993
Abstract
Hepatitis B virus (HBV) infection represents a worldwide health problem. For the prophylaxis and prevention of HBV infection, we have investigated on the production of mouse and human chimeric antibody which can bind to the pre-S2 surface antigen of HBV. The chimeric antibody genes were constructed by combining the mouse immunoglobulin heavy and light chain variable region (VH and VL) genes with human rl and k constant region genes, respectively, which was then cloned into pSV2neo and pSV2hygro to yield expression plasmids pHS2-neo and pLS2-hygro, respectively. The plasmids were sequentially electroporated into murine non-Ig-producing hybridoma (SP2/0) cells, and transfecto-mas secreting chimeric antibody were identified by ELISA. The chimeric antibodies were purified and characterized by 10% SDS-PAGE and western blot analysis. These results (demonstrated that the transfectomas functionally express and secrete chimeric antibody) which can bind to the pre-S2 surface antigen of HBV. The chimeric antibody may be useful to the therapy of HBV infection.
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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