Detection of human immunodeficiency virus type 1 antibody using whole-cell viral lysate and recombinant protein in enzyme-linked immunosorbentassay systems

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Title
Detection of human immunodeficiency virus type 1 antibody using whole-cell viral lysate and recombinant protein in enzyme-linked immunosorbentassay systems
Author(s)
Myoung Lyeh Park; Mi Jin Sohn; Young Ik Lee
Bibliographic Citation
Korean Biochemical Journal, vol. 26, no. 2, pp. 114-117
Publication Year
1993
Abstract
A total of 575 serum specimens received for human immunodeficiency virus type 1 (HIV-1) antibody determination were tested prospectively by enzyme immunoassay with a whole-cell viral lysate (VL) (Genetic Systems Corp.), a synthetic peptide (SP) (United Biomedical, Inc.), and a recombinant protein (RCP) (Syva Co.). Concordance of all three antigens was noted for 559 of 575 (97.2%) specimens tested. Of the specimens tested, 90 (15.7%) were positive and 469 (84.3%) were negative. Retrospective testing by SP and RCP of 86 specimens indeterminate for HIV-1 antibody by VL, Western blot (immunoblot), and immunofluorescence was also performed. The results of both phases of this study indicate that the specificity of the three antigens is RCP greater than SP greater than VL. As noted from the prospective phase, the sensitivities of the antigens appear to be equivalent, indicating that the RCP and SP systems could be used in place of, or to confirm, a VL enzyme immunoassay screening test.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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