Characterization of agglutinating monoclonal antibody against human red blood cells

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Title
Characterization of agglutinating monoclonal antibody against human red blood cells
Author(s)
Sang-Uk Nham; Suhn-Young Im; Kee-Soon Lee; Seo-Hee Cho; Young Ik Lee
Bibliographic Citation
Molecules and Cells, vol. 3, no. 2, pp. 221-224
Publication Year
1993
Abstract
As a first step to clone cDNAs encoding variable domains of the heavy and the light chain of hemagglutinating antibody, mouse monoclonal antibodies against human red blood cells were generated and the N-termini of the heavy and the light chain were sequenced. Ficoll-paque purified red blood cells were used as a antigen to generate hybridoma and nine clones were selected as positive clones by a hemagglutination assay. One clone (# 12) was selected as a strong antibody producer and monoclonal antibody secreted from the clone was purified to homogeneity. By amino acid analysis, N-terminal sequence of the heavy chain was shown to be Glu-Val-Gln-Leu-Gln-Gln-Ser-Gly-Pro and that of the light chain was Asp-He-Val-Met-Thr-Gln-Ser-Pro. The heavy chain sequence was identified as antibody subgroup Ha and that of the light chain was subgroup I or subgroup HI. Taken together, these results provided useful information to design oligonucleotides for cloning of antibody cDNA which is initial phase work toward developing immunodiagnostic using human whole blood.
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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