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- Title
- Characterization of agglutinating monoclonal antibody against human red blood cells
- Author(s)
- Sang-Uk Nham; Suhn-Young Im; Kee-Soon Lee; Seo-Hee Cho; Young Ik Lee
- Bibliographic Citation
- Molecules and Cells, vol. 3, no. 2, pp. 221-224
- Publication Year
- 1993
- Abstract
- As a first step to clone cDNAs encoding variable domains of the heavy and the light chain of hemagglutinating antibody, mouse monoclonal antibodies against human red blood cells were generated and the N-termini of the heavy and the light chain were sequenced. Ficoll-paque purified red blood cells were used as a antigen to generate hybridoma and nine clones were selected as positive clones by a hemagglutination assay. One clone (# 12) was selected as a strong antibody producer and monoclonal antibody secreted from the clone was purified to homogeneity. By amino acid analysis, N-terminal sequence of the heavy chain was shown to be Glu-Val-Gln-Leu-Gln-Gln-Ser-Gly-Pro and that of the light chain was Asp-He-Val-Met-Thr-Gln-Ser-Pro. The heavy chain sequence was identified as antibody subgroup Ha and that of the light chain was subgroup I or subgroup HI. Taken together, these results provided useful information to design oligonucleotides for cloning of antibody cDNA which is initial phase work toward developing immunodiagnostic using human whole blood.
- ISSN
- 1016-8478
- Publisher
- Korea Soc-Assoc-Inst
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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