DC Field | Value | Language |
---|---|---|
dc.contributor.author | Y H Yang | - |
dc.contributor.author | I K Kim | - |
dc.contributor.author | Hyang Sook Yoo | - |
dc.contributor.author | Dong Uk Kim | - |
dc.contributor.author | I S Sohn | - |
dc.contributor.author | K S Song | - |
dc.contributor.author | Y W Park | - |
dc.date.accessioned | 2017-04-19T08:44:18Z | - |
dc.date.available | 2017-04-19T08:44:18Z | - |
dc.date.issued | 1993 | - |
dc.identifier.issn | 0494-4755 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3275 | - |
dc.description.abstract | Prenatal determination of fetal 狀x is extremely important in the management of X-1 inked disorders such as Duchenne muscular, dystrophy, Hemophilia A and B, and the Leach-Nyhan syndrome. Previously fetal sex determination relied on karyotyping chorionic villus cell or amniocyte. Standard karyotyping depends on obtaining multiple h增h quality me-tapha效s from cells cultured for days to weeks before analysis. Polymerase chain reaction is a rapid and sensitive method to analyze a specific DNA sequence. Polymerase chain reaction can detect the DNA contained small amount of amniotic fluid, a single chorionic villus, a single cell equivalent. The result of polymerase chain reaction can be obtained in 4 to 6 hours. In this study, prenatal diagnosis for kx determination was performed by the use of cloned Y chromosome-speciflc DNA probes (Yl-1, Yl-2 and Yl-5, Yl-6) in 6 humdn lymphocyte, 11 chorionic villi sampling, 7 amniocentesis, 11 placental biopsy. Bright Y-specific band identified in all male DNA. AU results were verified by karyotyping. Y-specific sequences were amplified from all male DNA samples. Futher application of this technique,the analysis of sex-reversed individuals (46, XX males and 4^,XY females) and patients with other types of sex-chromosomal anomalies has led to the development and refinement of the deletioji map. "Die ina^sb of ^regiMnt peripheral blood for determin^tton bf fetal sex and fetal genetic dUonfers and prei변pjmtja^ion genetio diagnosis may be possible. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Molecular biological approach for analysis of fetal sex chromosomal DNA and its clinical application for prenatal genetic diagnosis = 분자생물학적방법에 의한 태아의 성염색체분석과 이의 산전 유전질환 진단응용 | - |
dc.title.alternative | Molecular biological approach for analysis of fetal sex chromosomal DNA and its clinical application for prenatal genetic diagnosis | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of Obstetrics and Gynecology | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 783 | - |
dc.citation.startPage | 773 | - |
dc.citation.volume | 36 | - |
dc.contributor.affiliatedAuthor | Hyang Sook Yoo | - |
dc.contributor.affiliatedAuthor | Dong Uk Kim | - |
dc.contributor.alternativeName | 양영호 | - |
dc.contributor.alternativeName | 김인규 | - |
dc.contributor.alternativeName | 유향숙 | - |
dc.contributor.alternativeName | 김동욱 | - |
dc.contributor.alternativeName | 손인숙 | - |
dc.contributor.alternativeName | 송경순 | - |
dc.contributor.alternativeName | 박용원 | - |
dc.identifier.bibliographicCitation | Korean Journal of Obstetrics and Gynecology, vol. 36, no. 6, pp. 773-783 | - |
dc.description.journalClass | N | - |
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