Production of poly-β-hydroxybutyrate and measurement of related enzyme activities in Alcaligenes eutrophus

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Title
Production of poly-β-hydroxybutyrate and measurement of related enzyme activities in Alcaligenes eutrophus
Author(s)
In Young Lee; Soo Wan Nam; Eun Soo Choi; Ho Nam Chang; Young Hoon Park
Bibliographic Citation
Journal of Fermentation Bioengineering, vol. 76, no. 5, pp. 416-418
Publication Year
1993
Abstract
Activities of β-ketothiolase (KT) and glucose-6-phosphate dehydrogenase (GPDH) were determined during the fermentation of Alcaligenes eutrophus NCIMB 11599 at three different values of C/N ratio. As the C/N ratio increased the cell growth decreased due to the limitation of nitrogen, while the cellular poly-β-hydroxybutyrate (PHB) content increased. At an initial C/N ratio of 32.8, the cellular PHB content reached 90.0% after 32 h cultivation. The specific activity of β-ketothiolase in each fermentation was the highest immediately after exhaustion of the nitrogen source. Specific activities of β-ketothiolase and glucose-6-phosphate dehydrogenase were maintained stably within the tested range of C/N ratio even after the N-source was completely exhausted.
ISSN
0385-6380
Publisher
Soc Bioscience Bioengineering Japan
DOI
http://dx.doi.org/10.1016/0922-338X(93)90037-9
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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