Production kinetics and stability of a transfectoma cell line secreting murine/human chimeric antibody

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dc.contributor.authorByung Rae Jin-
dc.contributor.authorChun Jeih Ryu-
dc.contributor.authorHyo Jeong Hong-
dc.contributor.authorMoon Hi Han-
dc.coverage.temporal20180101-20181231-
dc.date.accessioned2017-04-19T08:44:21Z-
dc.date.available2017-04-19T08:44:21Z-
dc.date.issued1993-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3294-
dc.description.abstractWe have developed a transfectomacell line H69K that secrets secreting chimeric antibody with specificity for the pre-S2 surface antigen by the introductionof the chimeric heavy and light chain genes into murine SP2/0 hybridoma cells. In order to apply the production kineticsto the large-scale production of chimeric antibody, the cell growth, productivity and stability of the cell line have beencharacterized. The results showed that the specific growth rate of the cells was almost the same in both the 5% serum medium and the serum free medium,but the specific productivity was higher in the serum free medium. The productivity also increa\-sed during the stationaryphase of the culture, suggesting that the kinetics of the chimeric antibody-production by the transfectoma cell line isassociated with growth phase. The transfec\-toma cell line was stable for three months even without selection and thereafterslightly lost the productivity. Ribonuclease protection assay of the heavy and light chain mRNA from the long-term culturesrevealed that decrease in the productivity may be due to the loss of the Ig genes in some cells. The informations obtainedfrom this study will be useful in the large-scale production of the chimericantibody by this transfectoma cell line.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleProduction kinetics and stability of a transfectoma cell line secreting murine/human chimeric antibody-
dc.title.alternativeProduction kinetics and stability of a transfectoma cell line secreting murine/human chimeric antibody-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number3-
dc.citation.endPage237-
dc.citation.startPage233-
dc.citation.volume3-
dc.contributor.affiliatedAuthorChun Jeih Ryu-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.contributor.affiliatedAuthorMoon Hi Han-
dc.contributor.alternativeName진병래-
dc.contributor.alternativeName류춘제-
dc.contributor.alternativeName홍효정-
dc.contributor.alternativeName한문희-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 3, no. 3, pp. 233-237-
dc.description.journalClassY-
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