DC Field | Value | Language |
---|---|---|
dc.contributor.author | Byung Rae Jin | - |
dc.contributor.author | Chun Jeih Ryu | - |
dc.contributor.author | Sung Sup Park | - |
dc.contributor.author | Uk NangGung | - |
dc.contributor.author | Hyo Jeong Hong | - |
dc.contributor.author | Moon Hi Han | - |
dc.date.accessioned | 2017-04-19T08:44:21Z | - |
dc.date.available | 2017-04-19T08:44:21Z | - |
dc.date.issued | 1993 | - |
dc.identifier.issn | 0161-5890 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3295 | - |
dc.description.abstract | The cloning, expression and characterization of a murine-human chimeric antibody with specificity for the pre-S2 surface antigen (Ag) of hepatitis B virus (HBV) is described. The heavy and light chain variable region (VH and VL) genes encoding the murine monoclonal antibody (mAb) were isolated and combined with human γ 1 and κ constant region genes, respectively. The expression vectors containing the chimeric heavy and light chain genes were sequentially electroporated into murine Sp2/0 hybridoma cells and transfectomas secreting chimeric antibody were isolated. The chimeric antibody was purified and characterized by ELISA, Western analysis and competition immunoassay, demonstrating that the transfectoma functionally express and secrete murine-human chimeric antibody which retained the specificity and affinity of the parental murine mAb. | - |
dc.publisher | Elsevier | - |
dc.title | Cloning, expression and characterization of a murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus | - |
dc.title.alternative | Cloning, expression and characterization of a murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus | - |
dc.type | Article | - |
dc.citation.title | Molecular Immunology | - |
dc.citation.number | 18 | - |
dc.citation.endPage | 1654 | - |
dc.citation.startPage | 1647 | - |
dc.citation.volume | 30 | - |
dc.contributor.affiliatedAuthor | Chun Jeih Ryu | - |
dc.contributor.affiliatedAuthor | Sung Sup Park | - |
dc.contributor.affiliatedAuthor | Hyo Jeong Hong | - |
dc.contributor.affiliatedAuthor | Moon Hi Han | - |
dc.contributor.alternativeName | 진병래 | - |
dc.contributor.alternativeName | 류춘제 | - |
dc.contributor.alternativeName | 박성섭 | - |
dc.contributor.alternativeName | 남궁욱 | - |
dc.contributor.alternativeName | 홍효정 | - |
dc.contributor.alternativeName | 한문희 | - |
dc.identifier.bibliographicCitation | Molecular Immunology, vol. 30, no. 18, pp. 1647-1654 | - |
dc.identifier.doi | 10.1016/0161-5890(93)90438-H | - |
dc.description.journalClass | Y | - |
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