Molecular properties of recombinant human alpha1-antitrypsin produced in Escherichia coli and in vitro translation system

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dc.contributor.authorKee Nyung Lee-
dc.contributor.authorHwa Soo Shin-
dc.contributor.authorKi Sun Kwon-
dc.contributor.authorSang Dai Park-
dc.contributor.authorMyeong Hee Yu-
dc.date.accessioned2017-04-19T08:44:21Z-
dc.date.available2017-04-19T08:44:21Z-
dc.date.issued1993-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3298-
dc.description.abstractThe cDNA encoding human al-antitrypsin (alAT) was expressed in Escherichia coli upto ?40% of total cellular proteins. The recombinant alAT carrying the Z type mutation (Glu342?>Lys) were accumulated as aggregates inside the E. coli cells while the wild type alAT were produced as a soluble form. The aggregation caused by the Z mutation became more significant as the growth temperature increased from 30 t to 37 t. A protein folding assay of alAT on SDS-polyaciylamide gel electrophoresis revealed that the Z polypeptides translated in vitro were in a conformation which could not bind elastase but rather served as a substrate for the protease. The results are consistent with the previously known Z type defects, aggregation inside the hepatocytes of patients and temperature-dependent oli-’ gomerization of the purified Z protein, ^rhe E. coli expression system and the folding (assay of in vitro translation product develoj)ed in the present study may be further explored / for characterization of other genetic variants of alAT.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleMolecular properties of recombinant human alpha1-antitrypsin produced in Escherichia coli and in vitro translation system-
dc.title.alternativeMolecular properties of recombinant human alpha1-antitrypsin produced in Escherichia coli and in vitro translation system-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number1-
dc.citation.endPage74-
dc.citation.startPage71-
dc.citation.volume3-
dc.contributor.affiliatedAuthorKee Nyung Lee-
dc.contributor.affiliatedAuthorKi Sun Kwon-
dc.contributor.affiliatedAuthorMyeong Hee Yu-
dc.contributor.alternativeName이기녕-
dc.contributor.alternativeName신화수-
dc.contributor.alternativeName권기선-
dc.contributor.alternativeName박상대-
dc.contributor.alternativeName유명희-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 3, no. 1, pp. 71-74-
dc.description.journalClassY-
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Aging Convergence Research Center > 1. Journal Articles
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