High frequency somatic embryogenesis and plant regeneration in tissue cultures of various genotypes of maize(Zea mays L.) = 다양한 계통의 옥수수에서 체세포배발생을 통한 효율적인 식물체 재분화

Cited 0 time in scopus
Metadata Downloads
Title
High frequency somatic embryogenesis and plant regeneration in tissue cultures of various genotypes of maize(Zea mays L.) = 다양한 계통의 옥수수에서 체세포배발생을 통한 효율적인 식물체 재분화
Author(s)
Won Joong Chung; Nam Hi Song; Sung Rsn Min; Myung K. Kim; Kwang-W. Lee; Jang Ryol Liu
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 20, no. 4, pp. 221-226
Publication Year
1993
Abstract
Somatic embryogenesis and plant regeneration was obtained from immature inflorescence segments of maize (Zea mays L.). Immature tassels and ears (0.5-3.0 cm) were excised, cut into segments (1 - 2 mm), and place onto media supplemented with varying concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), sucrose and l-proline. After 2-3 weeks, the segments produced compact, nodular, white, embryogenic callus as well as soft, granular, friable, non-embryogenic callus. Embryogenic callus further developed somatic embryos with distinct scutellar- and coleoptile-like structures, which produced normal, fertile plants with no apparent morphological abnormalities. Maximum embryogenic callus formation was observed when segments, cut from 0.6-1.0 cm inflorescences, were cultured on Murashige and Skoog (MS) medium with 2 mg/l 2,4-D, 3% sucrose, and 24 mM l-proline. Although embryogenic callus production was observed in all inbreds tested, significant genotype effects were apparent. The inbreds A619, H99, LH39, LH39, MBS501, MS71, Oh43, U94 and U98 all displayed high frequencies of embryogenic callus production (45-80% of cultured segments) and plant regeneration (up to 50% of callus pieces transferred). Thus, immature inflorescences can be used as explants for high frequency somatic embryogenesis and plant regeneration from several commercially-important maize inbreds.
Keyword
immature earsimmature tasselsplant tissue culturezea mays
ISSN
1015-5880
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.