DC Field | Value | Language |
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dc.contributor.author | Jong Hoon Park | - |
dc.contributor.author | Jae-Chan Song | - |
dc.contributor.author | Myung Hee Kim | - |
dc.contributor.author | Dae Sil Lee | - |
dc.contributor.author | Chul Ho Kim | - |
dc.date.accessioned | 2017-04-19T08:44:27Z | - |
dc.date.available | 2017-04-19T08:44:27Z | - |
dc.date.issued | 1994 | - |
dc.identifier.issn | 1350-0872 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3345 | - |
dc.description.abstract | Large restriction fragments of genomic DNA from Micrococcus sp. Y-1 were separated by pulsed-field gel electrophoresis (PFGE). Since Micrococcus sp. Y-1 has a G+C content of approximately 70%, restriction fragments were obtained by digesting chromosomal DNA with endonucleases which recognize A+T-rich sequences. Five enzymes, SspI, SpeI, XbaI, HpaI and EcoRI, were used for generation of distinctly separated fragments in the size range 100-500 kb. No site for DraI was detected. In contrast, sites for 8-base-recognizing enzymes, but not for NotI and SfiI, were frequent. The genome size of Micrococcus sp. Y-1 was determined from restriction fragments separated by PFGE, and was estimated to be approximately 4061 kb. Partial digestion experiments revealed the order of the six SspI fragments on the chromosome. | - |
dc.publisher | Microbiology Soc | - |
dc.title | Determination of genome size and a preliminary physical map an extreme alkaliphile Micrococcus sp. Y-1 by pulsed-field gel electrophoresis | - |
dc.title.alternative | Determination of genome size and a preliminary physical map an extreme alkaliphile Micrococcus sp. Y-1 by pulsed-field gel electrophoresis | - |
dc.type | Article | - |
dc.citation.title | Microbiology-SGM | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 2250 | - |
dc.citation.startPage | 2247 | - |
dc.citation.volume | 140 | - |
dc.contributor.affiliatedAuthor | Jong Hoon Park | - |
dc.contributor.affiliatedAuthor | Myung Hee Kim | - |
dc.contributor.affiliatedAuthor | Dae Sil Lee | - |
dc.contributor.affiliatedAuthor | Chul Ho Kim | - |
dc.contributor.alternativeName | 박종훈 | - |
dc.contributor.alternativeName | 송재찬 | - |
dc.contributor.alternativeName | 김명희 | - |
dc.contributor.alternativeName | 이대실 | - |
dc.contributor.alternativeName | 김철호 | - |
dc.identifier.bibliographicCitation | Microbiology-SGM, vol. 140, pp. 2247-2250 | - |
dc.identifier.doi | 10.1099/13500872-140-9-2247 | - |
dc.subject.keyword | alkaliphile | - |
dc.subject.keyword | genome size | - |
dc.subject.keyword | micrococcus sp. | - |
dc.subject.local | alkaliphile | - |
dc.subject.local | genome size | - |
dc.subject.local | micrococcus sp. | - |
dc.description.journalClass | Y | - |
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