Expression and secretion of human lipocortin-I by promoter and signal sequence from Saccharomyces diastaticus

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Title
Expression and secretion of human lipocortin-I by promoter and signal sequence from Saccharomyces diastaticus
Author(s)
Soo Wan Nam; Byung Moon Kim; Bong Hyun Chung; Dae Ook Kang; Jong Seog Ahn
Bibliographic Citation
Biotechnology Letters, vol. 16, no. 9, pp. 897-902
Publication Year
1994
Abstract
For the secretion of human lipocortin-1 (LC-1) in yeast, a expression and secretion vector was constructed by using the promoter and signal sequence of glucoamylase gene (STA1) of Saccharomyces diastaticus. After the cDNA of human LC-1 was ligated with the secretion vector, the resulting hybrid plasmid was transformed into S. diastaticus. When the recombinant S. diastaticus was cultivated in YPD medium, LC-1 was expressed and secreted into the extracellular medium, yielding LC-1 protein at a concentration of 2.5 g/mL.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00128621
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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