Effect of toluene-permeabilization on oxidation of d-sorbitol to l-sorbose by Gluconobacter suboxydans cells immobilized in calcium alginate

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Title
Effect of toluene-permeabilization on oxidation of d-sorbitol to l-sorbose by Gluconobacter suboxydans cells immobilized in calcium alginate
Author(s)
Young-Min Park; Eui Sung Choi; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 16, no. 4, pp. 345-348
Publication Year
1994
Abstract
The effect of permeabilization of G. suboxydans cells with toluene on the oxidation of D-sorbitol to L-sorbose was investigated. Treatment of the cells with 10% toluene resulted in a three fold increase in the specific sorbitol dehydrogenase activity and a two fold increase in the efficiency of D-sorbitol conversion to L-sorbose of the free cell suspension. When the permeabilized cells were immobilized in calcium alginate, the operational stability during air-lift reactor operation was also found to increase with up to three times longer half-life(44 days) of catalytic activity compared with immobilized intact cells.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00245048
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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