Isolation of thermostable D-hydantoinase-producing thermophilic Bacillus sp SD-1

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Title
Isolation of thermostable D-hydantoinase-producing thermophilic Bacillus sp SD-1
Author(s)
Seung Goo LeeDong Chul Lee; Moon Hee Sung; Hak-Sung Kim
Bibliographic Citation
Biotechnology Letters, vol. 16, no. 5, pp. 461-466
Publication Year
1994
Abstract
A thermophilic bacterium which showed highest thermostability and activity of the hydantoinase was isolated from 1,000 thermophiles and identified to be Bacillus sp. SD-1 according to morphological and physiological characteristics. The optimal growth temperature of the bacterium was about 60°C. The hydantoinase of Bacillus sp. SD-1 was strictly D-specific, and optimal pH and temperature were determined to be about 8.0 and 70°C, respectively. The D-hydantoinase was stable upto 70°C, and half-life of the enzyme was about 20 min at 80°C.
Keyword
hydantoin derivativebacillusbacterium isolationthermophilic bacteriumbacillus sp.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF01023326
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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