Secretion of human growth hormone from mammary gland of transgenic mice = 형질전환동물의 유선조직으로부터 인간 성장호르몬의 분비

Abstract

The human growth hormone (hGH) gene under the control of the rat ^-casein promoter gene was designed to produce transgenic mouse expressed hGH gene in only mammary gland. One hundred seventy two eggs microinjected were transferred to the oviducts of pseudopregnants and 43 offspring were delivered. By Southern blotting hybridization, 3 were transgenic with rat -casein /hGH gene. The copy numbers of three transgenic founder were 1, 5, and 15, respectively. A radioimmunoassay was developed to quantitate the amount of expression of the hGH gene m mammary gland of transgenic mice. The amount of hGH was 13.3 ng /ml in the lactating rrulk of one transgenic line, showing predominantly higher than 3.0 ng /ml in milk of control mice. Therefore, our findings suggested that 0-casein promoter may induce the tissue specific expression of structural gene.