Detection of β-gluosidase activity in polyacrylamide gels with esculin as substrate
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- Title
- Detection of β-gluosidase activity in polyacrylamide gels with esculin as substrate
- Author(s)
- Ki Sun Kwon; J Lee; H G Kang; Y C Hah
- Bibliographic Citation
- Applied and Environmental Microbiology, vol. 60, no. 12, pp. 4584-4586
- Publication Year
- 1994
- Abstract
- β-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by β-glucosidase action reacts with ferric ion to produce a black band, corresponding to the β-glucosidase, against the transparent background.
- ISSN
- 0099-2240
- Publisher
- Amer Soc Microb
- Type
- Article
- Appears in Collections:
- Aging Convergence Research Center > 1. Journal Articles
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