Detection of β-gluosidase activity in polyacrylamide gels with esculin as substrate

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Title
Detection of β-gluosidase activity in polyacrylamide gels with esculin as substrate
Author(s)
Ki Sun Kwon; J Lee; H G Kang; Y C Hah
Bibliographic Citation
Applied and Environmental Microbiology, vol. 60, no. 12, pp. 4584-4586
Publication Year
1994
Abstract
β-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by β-glucosidase action reacts with ferric ion to produce a black band, corresponding to the β-glucosidase, against the transparent background.
Keyword
esculinenzyme substrate complexpolyacrylamide gel electrophoresis
ISSN
0099-2240
Publisher
Amer Soc Microb
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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