Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme II and analyses of the deduced protein sequence

Abstract

The complete nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme II (EII(Man)) was determined. The gene consisted of 2052 base pairs encoding a protein of 683 amino acid residues; the molecular mass of the protein subunit was calculated to be 72570 Da. The N-terminal hydrophilic domain of EII(Man) showed 39.7% homology with a C-terminal hydrophilic domain of Escherichia coli glucose-specific enzyme II (EII(Glc)). Similar homology was shown between the C-terminal sequence of EII(Man) and the E. coli glucose-specific enzyme III (EIII(Glc)), or the EIII-like domain of Streptococcus mutans sucrose-specific enzyme II. Sequence comparison with other EIIs showed that EII(Man) contained residues His-602 and Cys-28 which were homologous to the potential phosphorylation sites of EIII(Glc), or EIII- like domains, and hydrophilic domains (IIB) of several EIIs, respectively.