Production of normal calves after transfer of IVF-derived bovine embryos = 체외수정란 유래의 송아지 생산

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dc.contributor.authorYong Mahn Han-
dc.contributor.authorChul Sang Lee-
dc.contributor.authorJ H Lee-
dc.contributor.authorSun Jung Kim-
dc.contributor.authorS T Shin-
dc.contributor.authorD H Kim-
dc.contributor.authorH T Lee-
dc.contributor.authorB H Chung-
dc.contributor.authorK S Chung-
dc.contributor.authorY S Kim-
dc.contributor.authorY H Kim-
dc.contributor.authorK S Lee-
dc.contributor.authorK K Kim-
dc.contributor.authorY S Hwang-
dc.contributor.authorKyung Kwang Lee-
dc.date.accessioned2017-04-19T08:44:30Z-
dc.date.available2017-04-19T08:44:30Z-
dc.date.issued1994-
dc.identifier.issnI000-0006-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3371-
dc.description.abstractTo verify in vivo viability of I VF-derived bovine embryos, morula and blastocysts that developed from in vitro matured and fertilized ova were transferred to the uteri of recipient cows and normal calves were produced. To produce I VF-derived bovine morula or blastocysts, ova matured and fertilized in vitro were cultrued in culture medium for 7 ?8 days at 39 °C under the humidified atmosphere of 5% CO2. Two different culture systems, a co-culture system with TCM-199 and bovine epithelial cells (BOEC) and CRlaa without somatic cell support, were compared. Cleavage rates to 2?8 cell stage and developmental rates of IVF-derived bovine embryos to blastocyst stage were not different between co-cultrue system (51.3 and 14.0%) and CRlaa medium (60.4 and 22.1%), respectively. Embryos were classified into three grades by embryo quality and then one or two embryos in higher quality (A and B grades) were transferred to the uterus of recipients. In this study Korean Native calf was first born after transfer of I VF-derived embryos. Total four live calves were normally developed to term from IVF-derived bovine blastocysts and one female fetus was stilbborn approximately 8 months of gestation, but there was no pregnancy after transfer of morula. Therfore, normal calves could be produced after transfer of I VF-derived bovine embryos cultured in CRlaa medium without somatic cell support. In addition, our results suggest that in transfer of I VF-derived bovine embryos blastocyst stage is better than morula.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleProduction of normal calves after transfer of IVF-derived bovine embryos = 체외수정란 유래의 송아지 생산-
dc.title.alternativeProduction of normal calves after transfer of IVF-derived bovine embryos-
dc.typeArticle-
dc.citation.titleReproductive & Developmental Biology-
dc.citation.number1-
dc.citation.endPage13-
dc.citation.startPage7-
dc.citation.volume18-
dc.contributor.affiliatedAuthorYong Mahn Han-
dc.contributor.affiliatedAuthorChul Sang Lee-
dc.contributor.affiliatedAuthorSun Jung Kim-
dc.contributor.affiliatedAuthorKyung Kwang Lee-
dc.contributor.alternativeName한용만-
dc.contributor.alternativeName이철상-
dc.contributor.alternativeName이정호-
dc.contributor.alternativeName김선정-
dc.contributor.alternativeName신상태-
dc.contributor.alternativeName김동훈-
dc.contributor.alternativeName이훈택-
dc.contributor.alternativeName정병헌-
dc.contributor.alternativeName정길생-
dc.contributor.alternativeName김영수-
dc.contributor.alternativeName김영훈-
dc.contributor.alternativeName이곤세-
dc.contributor.alternativeName김교국-
dc.contributor.alternativeName황윤식-
dc.contributor.alternativeName이경광-
dc.identifier.bibliographicCitationReproductive & Developmental Biology, vol. 18, no. 1, pp. 7-13-
dc.description.journalClassN-
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