Isolation and characterization of a naphthalene-degrading strain, Alcaligenes sp. A111 = Naphthalene 분해균주 Alcaligenes sp. A111의 분리 및 특성

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Title
Isolation and characterization of a naphthalene-degrading strain, Alcaligenes sp. A111 = Naphthalene 분해균주 Alcaligenes sp. A111의 분리 및 특성
Author(s)
Hee-Mock Oh; Jung Hyun Kang; Chang Ho Lee; Chan Sun Park; Sung Ku Ahn; Byung Dae Yoon; Yung Hee Kho
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 22, no. 4, pp. 423-429
Publication Year
1994
Abstract
A bacterial strain which formed a distinct colony on agar plate containing naphthalene as a vapor phase and grew well ina liquid minimal medium was isolated and identified as Alcaligenes sp. A111. Optimum temperature and pH for the cultivation of Alcaligenes sp. A111 were 30\cirC and 7.0, respectively. Cell growth increased dramatically from 12 hours after inoculation and revealed a stationary phase at about 48 hours. Relative growth rate (μ')increased hyperbolically depending on the conceration of naphthalene up to 500 ppm and reached to the maximum value pf 2.8/day, but μ' didn't change within a range of 500~4000 ppm naphthalene. NH4Cl or NH4NO3 was preferrd as a nitrogen source and a P : N ratio by weight og 6 : 1 was favorable to cell growth. Alcaligenes sp. A111 utilized the intermediates of degradation of naphthalene and showed tolerance to benzene, toluene, and octane. therefore, it is suggested that Alcaligenes sp. A111 could be effectively used for the biological treatment of wastewater containing naphthalene in the presence of some aromatic compounds.
Keyword
Alcaligenes sp. A111naphthalenebiodegradationisolation
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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