Overexpression and purification of full-length acidic fibroblast growth factor in E. coli

Cited 4 time in scopus
Metadata Downloads
Title
Overexpression and purification of full-length acidic fibroblast growth factor in E. coli
Author(s)
Sang Uk Nham; Hyo-Soon Kim; Young Ik Lee
Bibliographic Citation
Biotechnology Letters, vol. 16, no. 7, pp. 661-666
Publication Year
1994
Abstract
A full-length aFGF cDNA was cloned and inserted into a T7 expression vector for heterologous expression in E. coli. For high level production of aFGF, an optimal condition for overexpression (incubation at 42°C for 2 hours) and a simple purification procedure employing in vitro refolding and an affinity chromatography was established. The production yield was 40-50 μg/mg of inclusion body preparation and purified aFGF showed mitogenic activity.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00136467
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.