Effects of quality and developmental stage on the survival of IVF-derived bovine blastocysts cultured in vitro after freezing and thawing

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dc.contributor.authorYong Mahn Han-
dc.contributor.authorH Yamashina-
dc.contributor.authorN Koyama-
dc.contributor.authorKyung Kwang Lee-
dc.contributor.authorY Fukui-
dc.coverage.temporal20180629-20190428-
dc.date.accessioned2017-04-19T08:44:32Z-
dc.date.available2017-04-19T08:44:32Z-
dc.date.issued1994-
dc.identifier.issn0093-691X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3387-
dc.description.abstractFactors affecting viability of IVF-derived bovine blastocysts after freezing and thawing were investigated. A total of 1,101 ova matured and fertilized in vitro were cultured under 2 different conditions, 1) in TCM-199 on granulosa cell monolayers at 5% CO2 in air and 2) in synthetic oviduct fluid (SOF) medium without somatic cell support at 5% CO2, 5% O2, 90% N2. All blastocysts that developed from the 2 different culture systems were individually classified into 4 grades of embryo quality and were then frozen by conventional slow freezing. Developmental rates of the IVF-derived ova to blastocysts and the survival rates of the frozen-thawed blastocysts were not different between the SOF medium (16 and 49%) and the co-culture system (13 and 61%, respectively). Survival of frozen-thawed blastocysts was affected by embryo quality in both the SOF and co-culture systems (P<0.001). Blastocysts produced in vitro were also individually classified into 3 developmental stages and were then cultured for 3 d in the co-culture system with granulosa cells after freezing and thawing. There was a difference in the survival rate of frozen-thawed embryos between blastocyst developmental stages (early vs mid, P<0.05; mid vs expanded, P<0.01; early vs expanded, P<0.001). The post-thawing survival rate of blastocysts frozen at Day 7 (62%) of culture was higher compared with that of Day 8 (45%), but there was no difference in survival rate between Day 7 and 8 of culture. The results indicate that the quality and developmental stage of blastocysts are important factors influencing their survival after freezing and thawing.-
dc.publisherElsevier-
dc.titleEffects of quality and developmental stage on the survival of IVF-derived bovine blastocysts cultured in vitro after freezing and thawing-
dc.title.alternativeEffects of quality and developmental stage on the survival of IVF-derived bovine blastocysts cultured in vitro after freezing and thawing-
dc.typeArticle-
dc.citation.titleTheriogenology-
dc.citation.number4-
dc.citation.endPage654-
dc.citation.startPage645-
dc.citation.volume42-
dc.contributor.affiliatedAuthorYong Mahn Han-
dc.contributor.affiliatedAuthorKyung Kwang Lee-
dc.contributor.alternativeName한용만-
dc.contributor.alternativeNameYamashina-
dc.contributor.alternativeNameKoyama-
dc.contributor.alternativeName이경광-
dc.contributor.alternativeNameFukui-
dc.identifier.bibliographicCitationTheriogenology, vol. 42, no. 4, pp. 645-654-
dc.identifier.doi10.1016/0093-691X(94)90381-R-
dc.subject.keywordcattle-
dc.subject.keywordculture systems-
dc.subject.keyworddevelopmental stage-
dc.subject.keywordembryo quality-
dc.subject.keywordfreezing-
dc.subject.localCattle-
dc.subject.localcattle-
dc.subject.localculture systems-
dc.subject.localculture system-
dc.subject.localDevelopmental stage-
dc.subject.localdevelopmental stage-
dc.subject.localembryo quality-
dc.subject.localfreezing-
dc.description.journalClassY-
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