Rapid screening of an SH2-Containing gene using pcr amplification method

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Title
Rapid screening of an SH2-Containing gene using pcr amplification method
Author(s)
K M Lee; In Seong Choe; Mi Young Han
Bibliographic Citation
Biotechnology Techniques, vol. 8, no. 8, pp. 565-568
Publication Year
1994
Abstract
To isolate a novel gene that contains an SH2 domain, we devised a rapid and nonradioactive cDNA library screening method using polymerase chain reaction (PCR). For PCR amplification, we designed degenerate oligonucleotide primers from the multialigned DNA sequences of SH2 domains. This method offers an inexpensive and efficient approach for the isolation of clones of interest from cDNA libraries.
Keyword
Polymerase Chain ReactioncDNA LibraryPolymerase Chain Reaction AmplificationScreening MethodOligonucleotide Primer
ISSN
0951-208X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00152147
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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