Enhanced metal-affinity partitioning of genetically engineered hirudin variants in polyethylene glycol/dextran two-phase systems

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dc.contributor.authorBong Hyun Chung-
dc.contributor.authorJung Hoon Sohn-
dc.contributor.authorSang Ki Rhee-
dc.contributor.authorY K Chang-
dc.contributor.authorYoung Hoon Park-
dc.coverage.temporal20190422-20200421-
dc.date.accessioned2017-04-19T08:44:33Z-
dc.date.available2017-04-19T08:44:33Z-
dc.date.issued1994-
dc.identifier.issn0385-6380-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3391-
dc.description.abstractHirudin variants were constructed to exhibit an increased metal-binding affinity in an attempt to apply a metal-affinity partitioning process in a primary separation step for purification of hirudin. The hirudin variants were genetically engineered to contain additional surface-accessible histidines and produced by recombinant Saccharomyces cerevisiae. The partitioning behavior of these variants was compared with that of the wild type with a single surface-accessible histidine at position 51. Upon the addition of a small amount of Cu(II)IDA-PEG (Cu(II)iminodiacetic acid-polyethylene glycol) ligand to PEG/dextran two-phase systems, the hirudin variants with two or three surface-accessible histidines were more selectively partitioned into the PEG-rich phase than the wild type. Integrating protein engineering to metal-affinity partitioning offers the potential for general application of this technique to facilitate protein isolation, but the genetically engineered protein variants should be carefully constructed in a manner to minimize reduction of native protein activity.-
dc.publisherSoc Bioscience Bioengineering Japan-
dc.titleEnhanced metal-affinity partitioning of genetically engineered hirudin variants in polyethylene glycol/dextran two-phase systems-
dc.title.alternativeEnhanced metal-affinity partitioning of genetically engineered hirudin variants in polyethylene glycol/dextran two-phase systems-
dc.typeArticle-
dc.citation.titleJournal of Fermentation Bioengineering-
dc.citation.number1-
dc.citation.endPage79-
dc.citation.startPage75-
dc.citation.volume77-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.affiliatedAuthorJung Hoon Sohn-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.affiliatedAuthorYoung Hoon Park-
dc.contributor.alternativeName정봉현-
dc.contributor.alternativeName손정훈-
dc.contributor.alternativeName이상기-
dc.contributor.alternativeName장용근-
dc.contributor.alternativeName박영훈-
dc.identifier.bibliographicCitationJournal of Fermentation Bioengineering, vol. 77, no. 1, pp. 75-79-
dc.identifier.doi10.1016/0922-338X(94)90212-7-
dc.description.journalClassY-
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Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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