DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bong Hyun Chung | - |
dc.contributor.author | Jung Hoon Sohn | - |
dc.contributor.author | Sang Ki Rhee | - |
dc.contributor.author | Y K Chang | - |
dc.contributor.author | Young Hoon Park | - |
dc.coverage.temporal | 20190422-20200421 | - |
dc.date.accessioned | 2017-04-19T08:44:33Z | - |
dc.date.available | 2017-04-19T08:44:33Z | - |
dc.date.issued | 1994 | - |
dc.identifier.issn | 0385-6380 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3391 | - |
dc.description.abstract | Hirudin variants were constructed to exhibit an increased metal-binding affinity in an attempt to apply a metal-affinity partitioning process in a primary separation step for purification of hirudin. The hirudin variants were genetically engineered to contain additional surface-accessible histidines and produced by recombinant Saccharomyces cerevisiae. The partitioning behavior of these variants was compared with that of the wild type with a single surface-accessible histidine at position 51. Upon the addition of a small amount of Cu(II)IDA-PEG (Cu(II)iminodiacetic acid-polyethylene glycol) ligand to PEG/dextran two-phase systems, the hirudin variants with two or three surface-accessible histidines were more selectively partitioned into the PEG-rich phase than the wild type. Integrating protein engineering to metal-affinity partitioning offers the potential for general application of this technique to facilitate protein isolation, but the genetically engineered protein variants should be carefully constructed in a manner to minimize reduction of native protein activity. | - |
dc.publisher | Soc Bioscience Bioengineering Japan | - |
dc.title | Enhanced metal-affinity partitioning of genetically engineered hirudin variants in polyethylene glycol/dextran two-phase systems | - |
dc.title.alternative | Enhanced metal-affinity partitioning of genetically engineered hirudin variants in polyethylene glycol/dextran two-phase systems | - |
dc.type | Article | - |
dc.citation.title | Journal of Fermentation Bioengineering | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 79 | - |
dc.citation.startPage | 75 | - |
dc.citation.volume | 77 | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.affiliatedAuthor | Jung Hoon Sohn | - |
dc.contributor.affiliatedAuthor | Sang Ki Rhee | - |
dc.contributor.affiliatedAuthor | Young Hoon Park | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.contributor.alternativeName | 손정훈 | - |
dc.contributor.alternativeName | 이상기 | - |
dc.contributor.alternativeName | 장용근 | - |
dc.contributor.alternativeName | 박영훈 | - |
dc.identifier.bibliographicCitation | Journal of Fermentation Bioengineering, vol. 77, no. 1, pp. 75-79 | - |
dc.identifier.doi | 10.1016/0922-338X(94)90212-7 | - |
dc.description.journalClass | Y | - |
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