L-sorbose production from glucose and fructose using Zymomonas mobilis and Gluconobacter suboxydans in a two-stage fed-batch reactor

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Title
L-sorbose production from glucose and fructose using Zymomonas mobilis and Gluconobacter suboxydans in a two-stage fed-batch reactor
Author(s)
Won-Keuk Kim; Uck-Han Chun; Young-Min Park; Chul Ho Kim; Eui Sung Choi; Sang Ki Rhee
Bibliographic Citation
Process Biochemistry, vol. 29, pp. 277-283
Publication Year
1994
Abstract
A biological conversion of L-sorbose from the mixture of glucose and fructose was studied. D-Sorbitol was produced efficiently from fructose by permeabilized Zymomonas mobilis cells and then converted to L-sorbose by Gluconobacter suboxydans. The process for the production of L-sorbose from D-sorbitol by the resting cells of G. suboxydans was also optimized with respect to the substrate concentration, oxygen tension and cell density of the reaction medium. Gluconate produced from glucose by Z. mobilis seemed to act as an inhibitor in the conversion reaction of D-sorbitol into L-sorbose by G. suboxydans. A two-stage fed-batch process, therefore, was developed to minimize the inhibitory effect of gluconate on the conversion reaction.
ISSN
0032-9592
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0032-9592(94)80069-3
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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