High frequency plant regeneration from anther-derived cell suspension cultures via somatic embryogenesis in Catharanthus roseus

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Title
High frequency plant regeneration from anther-derived cell suspension cultures via somatic embryogenesis in Catharanthus roseus
Author(s)
Suk Weon Kim; Nam H Song; Kyung H Jung; Sang Soo Kwak; Jang Ryol Liu
Bibliographic Citation
Plant Cell Reports, vol. 13, pp. 319-322
Publication Year
1994
Abstract
A system for high frequency plant regeneration from cell suspension cultures in Catharanthus roseus is described. Calli were obtained from anthers cultured on Murashige and Skoog's medium supplemented with 1 mgl-1α-naphthaleneacetic acid and 0.1 mgl-1 kinetin. After the second subculture on solid medium, embryogenic callus was identified and transferred to liquid medium to initiate suspension cultures. Cells dispersed finely in the medium were subcultured at 14-day intervals. Upon plating onto the basal medium, yellowish compact colonies proliferated from the cells and more than 80% of them gave rise to somatic embryos. Subsequently, plantlets developed from the embryos. Both the plantlets and the source plants showed the normal somatic chromosome number of 2n=2x=16.
Keyword
anther cultureCatharanthus roseusplant regenerationsomatic embryogenesis
ISSN
0721-7714
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00232629
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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