Effect of amino acid substitution on the subtype specificity of preS2 epitope in hepatitis B surface antigen

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Title
Effect of amino acid substitution on the subtype specificity of preS2 epitope in hepatitis B surface antigen
Author(s)
Aeree Moon; Ki Sun Kwon; Myeong Hee Yu
Bibliographic Citation
Korean Biochemical Journal, vol. 27, no. 1, pp. 60-63
Publication Year
1994
Abstract
Antigenicity of the preS2 region of hepatitis B surface antigen was characterized by site-directed mutagenesis. Studies of serial deletions located the antigenic epitope between amino acid residues 132 and 143. Differences in the amino acid sequence between the adr and adu)2 subtypes occur at residues 132 and 141. Amino acid substitutions at residue 132 in the adr sequence did not affect antigenicity of the preS2 to 仕te monoclonal antibody H8, which is specific to the adr subtype preS2 sequence. However, substitutions at residue 141 did lead to a loss of antigenicity. A change of amino acid residue 141 from leucine 切 phenylalanine in 什? preS2 sequence of the adw2 subtype was sufficient for the sequence to be recognized by the monoclonal antibody H8. These results indicate that a single amino acid, Phe141, in the preS2 epitope is responsible for determining the subtype specificity recognized by the monoclonal an社body H8.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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