A metabolomics approach to identify factors influencing their α-glucosidase and hDPP-IV activity relative to chemical marker in adzuki bean (Vigna angularis (Willd.) Ohwi & H. Ohashi) cultivars = 팥(Vigna angularis (Willd.) Ohwi & H. Ohashi) 품종의 화학적 마커와 비교하여 항당뇨병 활성에 영향을 미치는 요인을 식별하기 위한 대사체학 접근법

Abstract

The metabolite changes in adzuki bean, as functional food, and their effects on its biological activity were monitored. The in adzuki bean cultivars were successfully distinguished by multivariate statistical analysis in electrospray ionization mode. As shown by the PCA scatter plot, volcano plot and box plot, the α-glucosidase exhibited significant chemical markers derived from 355.1271, 342.1189, 666.2219, and 1133.5381 m/z, whereas the hDPP-IV exhibited tentative chemical markers derived from 666.2220 and 342.1193 m/z. The adzuki bean extracts also inhibited α-glucosidase and hDPP-IV differently depending on the secondary metabolites composition. In detail, Milyang59, Haeoreum, Hongda, and Hongyeon inhibited α-glucosidase by 29.3, 16.6, 139.9, and 20.6% at 600 μg/mL, respectively, while Milyang59, Joongwon, Haeoreum and Hongmiin inhibited hDPP-IV by 15.0, 21.0, 15.0, and 20.0% at 600 μg/mL, respectively. Importantly, discrimination was shown across the cultivars of the adzuki bean, which correlated strongly with the azukisaponin VI and their derivatives in Milyang59 and Haeoreum.