Secretion of Bacillus endoglucanase in Saccharomyces cerevisiae by its own signal sequence

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Title
Secretion of Bacillus endoglucanase in Saccharomyces cerevisiae by its own signal sequence
Author(s)
Y J Han; Dae Ook Kang; S C Lee; Bo Yeon Kim; Hyun Hyo Suh; J M Kim; Tae Ick MheenJong Seog Ahn
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 4, no. 1, pp. 24-29
Publication Year
1994
Abstract
To examine whether the signal sequence of Bacillus endo-1, 4-glucanase can act functionally in a yeast, a lower eucaryote, two recombinant plasmids were constructed and introduced into Saccharomyces cerevisiae: recombinant plasmid pGCMC10 containing the complete signal sequence of Bacillus endoglucanase, and pGCMC11 without the signal sequence. Secretion of endoglucanase into culture medium was obtained with the yeast transformant containing plasmid pGCMC10. The secreted endoglucanase was glycosylated and was apparently processed to be about 36 kilodaltons (KDa) and 43KDa proteins. The glycosylated endoglucanase from yeast transformant was more thermostable than the nonglycosylated endoglucanase from Escherichia coli transformant.
Keyword
secretionBacillus endoglucanaseSaccharomyces cerevisiaesignal sequence
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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