Cited 0 time in
- Title
- Secretion of Bacillus endoglucanase in Saccharomyces cerevisiae by its own signal sequence
- Author(s)
- Y J Han; Dae Ook Kang; S C Lee; Bo Yeon Kim; Hyun Hyo Suh; J M Kim; Tae Ick Mheen; Jong Seog Ahn
- Bibliographic Citation
- Journal of Microbiology and Biotechnology, vol. 4, no. 1, pp. 24-29
- Publication Year
- 1994
- Abstract
- To examine whether the signal sequence of Bacillus endo-1, 4-glucanase can act functionally in a yeast, a lower eucaryote, two recombinant plasmids were constructed and introduced into Saccharomyces cerevisiae: recombinant plasmid pGCMC10 containing the complete signal sequence of Bacillus endoglucanase, and pGCMC11 without the signal sequence. Secretion of endoglucanase into culture medium was obtained with the yeast transformant containing plasmid pGCMC10. The secreted endoglucanase was glycosylated and was apparently processed to be about 36 kilodaltons (KDa) and 43KDa proteins. The glycosylated endoglucanase from yeast transformant was more thermostable than the nonglycosylated endoglucanase from Escherichia coli transformant.
- Keyword
- secretionBacillus endoglucanaseSaccharomyces cerevisiaesignal sequence
- ISSN
- 1017-7825
- Publisher
- Korea Soc-Assoc-Inst
- Type
- Article
- Appears in Collections:
- Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.