Selection of high yielding mutant strains for the antifungal antibiotics KRF-001 = 항진균물질 KRF-001의 고생산성 변이주 분리

Abstract

An impr ved method for the selective isolation of high-yielding mutant strains for the production of antifungal mtibiotic KRF-001 was investigated. The mutant strain U. V 4, which produces high titer of KRF-001, vas selected on the high potency agar plate after ultraviolet light irradiation. The U. V 4 strain prod iced 2-fold more KRF-001 than the mother strain in production media. Large scale fermen-ta仕on was)erformed using the U. V 4 strain in 100^ fermenter. The antifungal antibiotic KRF-001 secreted into :ulture broth was detected by HPLC in 24hrs of fermentation.