Classification and phylogenetic analysis of Acetobacter sp. HA by 5 S ribosomal RNA sequencing

Abstract

The strain Acetobacter sp. HA was isolated from Korean traditional fermented vinegar and defined as a new acidophilic Acetobacter species [Chun, H. S.，and Kim, S. J. (1993) J. MicrobioL Biotech, 5, 108-114]. Ribosomal 5 S RNA was isolated from Acetobacter sp. HA and sequenced by the direct chemical methods. Acetobacter sp. HA contained RNAs belonging to the 121 nucleotide type. The sequence of the cytoplasmic 5 S rRNA from Acetobacter sp. HA was compared to the known 5 S rRNA sequences of 11 strains of the genus Acetobacter and two strains of the Gluconobacter. Based on KnUC values, the Acetobacter sp. HA was phyloge-netically closer to Acetobacter aceti than to any other strains of Acetobacter or Gluconobacter,