Enzymatic synthesis of levan by Zymomonas mobilis levansucrase overexpressed in Escherichia coli

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Title
Enzymatic synthesis of levan by Zymomonas mobilis levansucrase overexpressed in Escherichia coli
Author(s)
Ki Bang Song; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 16, no. 12, pp. 1305-1310
Publication Year
1994
Abstract
Levansucrase gene from Zymomonas mobilis was expressed efficiently in Escherichia coli and the overproduced recombinant levansucrase amounted to 40% of the total cell protein. Using E. coli lysate, levan was synthesized in a sucrose-based medium enzymatically with the conversion yields of up to 46% from fructose liberated in 25 hrs of incubation. More levan was formed at lower temperatures in the reaction mixture, whereas higher temperatures were favoured for the accumulation of free fructose or short chain oligosaccharides.
Keyword
levanlevansucraseescherichia colisynthesiszymomonas mobilis
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00149637
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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