Expression of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae

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Title
Expression of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae
Author(s)
Dong-Hoon Lee; Jin-Byung Park; Jin-Ho Seo; Eui Sung Choi; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 16, no. 7, pp. 667-670
Publication Year
1994
Abstract
Fed-batch fermentations were performed to produce hirudin, an anticoagulant protein, from recombinant S. cerevisiae. The structural gene coding for hirudin was combined with the GAL10 promoter for controlled expression and the MFα1 signal sequence for secretion to the growth medium. Control of galactose concentration in a fed-batch mode of operation yielded 110 g/L of final cell density and 260 mg/L of maximum hirudin concentration in the medium, which corresponds to a 3.5-fold increase in cell density and a 4.1-fold enhancement in hirudin concentration compared with the batch fermentation.
Keyword
hirudinculture mediumfungus culturegene expressionrecombinant plasmidsaccharomyces cerevisiaesaccharomyces
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00136468
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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