Nucleotide and derived amino acid sequences of an extracellular sucrase gene (invB) of Zymomonas mobilis ZM1 (ATCC10988)

Abstract

DNA sequence analysis of a previously cloned 4.5 kb DNA fragment showed that the extracellular sucrase gene (invB) of Zymomonas mobilis was located in the 155 bp downstream of levansucrase gene (levU). The inuB gene had an open reading frame of 1242 bp and the deduced amino acid sequence was 413 residues with a molecular weight of 46107. The translated sequence of Z. mobilis inuB was in good agreement with the 10 N-terminal amino acid residues determined by amino acid sequencing. The amino acid sequence of sucrase showed low similarity with that of other sucrases, but higher similarity with that of levansucrases.