Identification of 4-demethylsterols from suspension cultured cells of Marchantia polymorpha L. = Marchantia polymorpha( 우산이끼 ) 현탁배양 세포내의 4-demethylsterol 들의 동정

Abstract

Five kinds of 4-demethylsterol were isolated from suspension cultured cells of a liverworth, Marchantia polymorpha. Four 4-demethylsterols among them were analyzed by a capillary gas chromatography-mass spectrometry and 500 MHz 1H-NMR, and characterized to be avenasterol, 24-methylene-cholesterol, stigmasterol, and campesterol. And the fifth 4-demethylsterol was characterized to be sitosterol by a capillary gas chromatography-mass spectrometry. The concentration of the 4-demethylsterols in the cell decreased in order of avenasterol gt stigmasterol gt 24-methylene-cholesterol gt campesterol gt sitosterol. When carbon skeleton and the oxidation state was compared with those of brassinosteroids, these 4-demethylsterols may be potent biosynthetic precursors of brassinosteroids in the cells.