자외선에 의한 면역반응의 억제를 회복시키는 면역조절물질을 생산하는 토양 Streptomyces sp.의 분리 및 동정

Abstract

Soil microorganisms producing immunomodulators that can restore ultraviolet B (UVB) radiation-induced suppression of the immune response were screened in vitro. Exposure of freshly isolated murine epidermal cells (EC) to 180 J/m2 of UVB radiation resulted in approximately 90% impairment of accessory cell function, as measured by their ability to support anti-CD3 monoclonal antibody-induced T-cell mitogenesis. When the culture supernatants of 150 actinomycete strains were examined for their capacity to prevent or repair the UVB-induced impairment of accessory cell function, 4 of them were identified to contain immunomodulators that can restore the decreased accessory cell function. The soil isolate that showed the most effective restorative activity, G40025, was selected and further characterized. Addition of 10mcl of the culture supernatant of G4002 grown in G-media to cultures of UVB-irradiated EC right after UVB-irradiation restored the decreased accessory cell function by 58%. The immunomodulator produced by G40025 appeared to be stabled at 100oC for 10 min. Taxonomical studies by cultural, morphological, and physiological characterization showed that the soil isolate, G4002, belongs to the genus Streptomyces.