DC Field | Value | Language |
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dc.contributor.author | Ho Jae Lee | - |
dc.contributor.author | Myung Chul Chung | - |
dc.contributor.author | Choong Hwan Lee | - |
dc.contributor.author | Hyo Kon Chun | - |
dc.contributor.author | Yung Hee Kho | - |
dc.date.accessioned | 2017-04-19T08:44:49Z | - |
dc.date.available | 2017-04-19T08:44:49Z | - |
dc.date.issued | 1995 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3530 | - |
dc.description.abstract | The Gelatinases (typeIV collagenases) are metalloproteinases that may play an important role in tumor invasion and metastasis. Progelatinase A was purified from a conditioned medium of T98G human glioblastoma cells. TIMP-2 complexed progelatinase A and free progelatinase A were separated by heparin affinity HPLC. The final product was homogeneous on SDS-PAGE, with a molecular weight of 64,000 daltons without reduction. TIMP-2 and free progelatinase A were separated from TIMP-2 complexed progelatinase A by reverse-phase HPLC in the presence of trifluoroacetic acid. TIMP-2 complexed progelatinase A was resistant to activation by p-aminophenyl mercuric acetate (APMA), and showed less than 20% of the activity of the TIMP-2 free active enzyme. TIMP-2 free progelatinase A was easily activated to the mature form with a molecular weight of 57,000 daltons by APMA and showed high activity compared to the TIMP-2 complexed enzyme. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Purification of progelatinase A (matrix metalloproteinase 2) and a tissue inhibitor of metalloproteinase-2 (TIMP-2) from T98G human glioblastoma cells | - |
dc.title.alternative | Purification of progelatinase A (matrix metalloproteinase 2) and a tissue inhibitor of metalloproteinase-2 (TIMP-2) from T98G human glioblastoma cells | - |
dc.type | Article | - |
dc.citation.title | BMB Reports | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 39 | - |
dc.citation.startPage | 33 | - |
dc.citation.volume | 28 | - |
dc.contributor.affiliatedAuthor | Ho Jae Lee | - |
dc.contributor.affiliatedAuthor | Myung Chul Chung | - |
dc.contributor.affiliatedAuthor | Choong Hwan Lee | - |
dc.contributor.affiliatedAuthor | Hyo Kon Chun | - |
dc.contributor.affiliatedAuthor | Yung Hee Kho | - |
dc.contributor.alternativeName | 이호재 | - |
dc.contributor.alternativeName | 정명철 | - |
dc.contributor.alternativeName | 이충환 | - |
dc.contributor.alternativeName | 전효곤 | - |
dc.contributor.alternativeName | 고영희 | - |
dc.identifier.bibliographicCitation | BMB Reports, vol. 28, no. 1, pp. 33-39 | - |
dc.subject.keyword | Matrix metalloproteinases | - |
dc.subject.keyword | TIMP | - |
dc.subject.keyword | glioblastoma | - |
dc.subject.local | Matrix metalloproteinases | - |
dc.subject.local | matrix metalloproteinase | - |
dc.subject.local | Matrix metalloproteinase | - |
dc.subject.local | matrix metalloproteinases | - |
dc.subject.local | TIMP | - |
dc.subject.local | glioblastoma | - |
dc.subject.local | Glioblastoma | - |
dc.description.journalClass | Y | - |
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