Purification of progelatinase A (matrix metalloproteinase 2) and a tissue inhibitor of metalloproteinase-2 (TIMP-2) from T98G human glioblastoma cells

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dc.contributor.authorHo Jae Lee-
dc.contributor.authorMyung Chul Chung-
dc.contributor.authorChoong Hwan Lee-
dc.contributor.authorHyo Kon Chun-
dc.contributor.authorYung Hee Kho-
dc.date.accessioned2017-04-19T08:44:49Z-
dc.date.available2017-04-19T08:44:49Z-
dc.date.issued1995-
dc.identifier.issn1225-8687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3530-
dc.description.abstractThe Gelatinases (typeIV collagenases) are metalloproteinases that may play an important role in tumor invasion and metastasis. Progelatinase A was purified from a conditioned medium of T98G human glioblastoma cells. TIMP-2 complexed progelatinase A and free progelatinase A were separated by heparin affinity HPLC. The final product was homogeneous on SDS-PAGE, with a molecular weight of 64,000 daltons without reduction. TIMP-2 and free progelatinase A were separated from TIMP-2 complexed progelatinase A by reverse-phase HPLC in the presence of trifluoroacetic acid. TIMP-2 complexed progelatinase A was resistant to activation by p-aminophenyl mercuric acetate (APMA), and showed less than 20% of the activity of the TIMP-2 free active enzyme. TIMP-2 free progelatinase A was easily activated to the mature form with a molecular weight of 57,000 daltons by APMA and showed high activity compared to the TIMP-2 complexed enzyme.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titlePurification of progelatinase A (matrix metalloproteinase 2) and a tissue inhibitor of metalloproteinase-2 (TIMP-2) from T98G human glioblastoma cells-
dc.title.alternativePurification of progelatinase A (matrix metalloproteinase 2) and a tissue inhibitor of metalloproteinase-2 (TIMP-2) from T98G human glioblastoma cells-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number1-
dc.citation.endPage39-
dc.citation.startPage33-
dc.citation.volume28-
dc.contributor.affiliatedAuthorHo Jae Lee-
dc.contributor.affiliatedAuthorMyung Chul Chung-
dc.contributor.affiliatedAuthorChoong Hwan Lee-
dc.contributor.affiliatedAuthorHyo Kon Chun-
dc.contributor.affiliatedAuthorYung Hee Kho-
dc.contributor.alternativeName이호재-
dc.contributor.alternativeName정명철-
dc.contributor.alternativeName이충환-
dc.contributor.alternativeName전효곤-
dc.contributor.alternativeName고영희-
dc.identifier.bibliographicCitationBMB Reports, vol. 28, no. 1, pp. 33-39-
dc.subject.keywordMatrix metalloproteinases-
dc.subject.keywordTIMP-
dc.subject.keywordglioblastoma-
dc.subject.localMatrix metalloproteinases-
dc.subject.localmatrix metalloproteinase-
dc.subject.localMatrix metalloproteinase-
dc.subject.localmatrix metalloproteinases-
dc.subject.localTIMP-
dc.subject.localglioblastoma-
dc.subject.localGlioblastoma-
dc.description.journalClassY-
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Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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