Expression of cholesteryl ester transfer protein cDNA using recombinant vaccinia viruses

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Expression of cholesteryl ester transfer protein cDNA using recombinant vaccinia viruses
M K Jang; B Y Ahn; T L Huh; Song Hae Bok; Y B Park
Bibliographic Citation
BMB Reports, vol. 28, pp. 216-220
Publication Year
cDNA for human cholesteryl ester transfer protein (CETP), a potent atherogenic plasma protein that redistributes the neutral lipids among lipoproteins, was expressed in recombinant vaccinia virus-infected cells (CV-1). Two insertion vectors regulated by different promoters were constructed. The vectors were introduced into human thymidine kinase-negative (TK^-) 143B cells infected with wild-type vaccinia virus (WR strain). Recombinant viruses were selected with 5-bromodeoxyuridine (BUdR) and X-gal and identified with DNA dot blot analysis (vSC11-CETP and vTM1-CETP). The CETP cDNA insert in the recombinant vaccinia virus genome was identified by Southern blot analysis. Transcription of CETP cDNA in CV-1 cells infected with recombinant vaccinia virus was monitored by Northern blot analysis using the CETP cDNA as a probe. Positive signals were detected at 1.8 kb in cells infected with vSC11-CETP and at 2.3 kb in cells infected with vTM1-CETP. The recombinant vaccinia virus-infected CV-1 cells were shown to produce functional CETP when the culture medium was subjected to the CETP assay.
Cholesteryl Ester Transfer Protein (CETP)Lipoprotein MetabolismVaccinia Virus Expression SystemGene Expression
Korea Soc-Assoc-Inst
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