Purification and characterization of a xylanase from Bacillus sp. KK-1

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dc.contributor.authorDae-Joon Kim-
dc.contributor.authorKi Hong Yoon-
dc.contributor.authorSeung Ho Kim-
dc.contributor.authorKi Haeng Cho-
dc.contributor.authorBon-Hong Min-
dc.date.accessioned2017-04-19T08:44:50Z-
dc.date.available2017-04-19T08:44:50Z-
dc.date.issued1995-
dc.identifier.issn1225-8687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3533-
dc.description.abstractA thermophilic Bacillus sp. strain KK-1 isolated from soil produced an extracellular xylanase. From the culture supernatant of Bacillus sp., the xylanase was purified to homogeneity by ammonium sulfate precipitation and DEAE-Sephadex A-50 chromatography. The molecular weight of the purified xylanase was estimated to be 45 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel permeation chromatography. The apparent values for xylanase, using oat spelt xylan and birchwood xylan as substrates, were 7.1 mg/ml and 3.2 mg/ml, and values were and , respectively. The xylanase hydrolyzed oat spelt xylan to mostly xylobiose, xylotriose, and xylose. The amino acid composition indicated that the xylanase contained high amounts of amino add residues of glutamic acid and glutamine (Glx) and aspartic acid and asparagine (Asx).-
dc.publisherKorea Soc-Assoc-Inst-
dc.titlePurification and characterization of a xylanase from Bacillus sp. KK-1-
dc.title.alternativePurification and characterization of a xylanase from Bacillus sp. KK-1-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number4-
dc.citation.endPage352-
dc.citation.startPage348-
dc.citation.volume28-
dc.contributor.affiliatedAuthorKi Hong Yoon-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.alternativeName김대준-
dc.contributor.alternativeName윤기홍-
dc.contributor.alternativeName김승호-
dc.contributor.alternativeName조기행-
dc.contributor.alternativeName민본홍-
dc.identifier.bibliographicCitationBMB Reports, vol. 28, no. 4, pp. 348-352-
dc.subject.keywordcellulase-free xylanase-
dc.subject.keywordthermophilic Bacillus sp.-
dc.subject.localcellulase-free xylanase-
dc.subject.localthermophilic Bacillus sp.-
dc.description.journalClassY-
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