Production of egg yolk antibody (IgY) against human placental DNA-dependent RNA polymerase II

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Title
Production of egg yolk antibody (IgY) against human placental DNA-dependent RNA polymerase II
Author(s)
Yoon Ik Lee; S S Surzycki; Young Ik Lee
Bibliographic Citation
BMB Reports, vol. 28, no. 1, pp. 27-32
Publication Year
1995
Abstract
Polyclonal antibodies against human DNA-dependent RNA polymerase II (HPP II) were generated from chicken egg yolk after immunization with RNA polymerase II as an antigen. The antibodies from egg yolk (IgY) were purified and characterized. IgY showed a specificity against DNA-dependent RNA polymerase II, and was a polyclonal antibody against 12 subunits of polymerase II. An amount of 0.35 mg of IgY was obtained freman HPP II-Sepharose affinity column using 10 eggs from a chicken immunized against RNA polymerase II as an antigen. These antibodies can be used for isolating the genes for RNA polymerase II components, and for in vitro transcription assays using HP-RNA polymerase II.
Keyword
Egg yolk Antibody (IgY)DNA-Dependent RNA polymerase-Ⅱ (DNA-RNAPⅡ)polyclonal antibodyIgY-purifications
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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