Anti-inflammatory and anti-oxidant effects of Zanthoxylum myriacanthum Wall. ex Hook. f. extract in RAW 264.7 macrophages = RAW 264.7 대식세포에서 Zanthoxylum myriacanthum Wall. ex Hook. f. 추출물의 항염증 및 항산화 효과

Abstract

Zanthoxylum species are used worldwide as medicines for immune function regulation, enteroprotective and hepatoprotective activity, as well as anti-inflammatory and anti-oxidant activity. However, the anti-inflammatory effects of the Zanthoxylum myriacanthum Wall. ex Hook. f. are unknown. Therefore, this study evaluated the anti-inflammatory efficacy of Zanthoxylum myriacanthum Wall. ex Hook. f. extracts (ZME) in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). ZME did not cause cytotoxicity in RAW 264.7 macrophages and significantly reduced nitric oxide production and the expression of inducible nitric oxide synthase induced by LPS. In addition, ZME inhibited the phosphorylation of p65, I kappa B (IκB), I kappa B kinase, and the degradation of IκB induced by LPS in RAW 264.7 macrophages. An analysis of reactive oxygen species (ROS) production using the DCF-DA probe showed that ZME inhibited ROS production induced by LPS. Moreover, ABTS and DPPH analysis revealed ZME to have antioxidant ability in RAW 264.7 macrophages. Therefore, ZME can inhibit LPS-induced inflammatory response and oxidative stress through the signaling pathway caused by the nuclear factor-kappa B transcription factor in RAW 264.7 macrophages. These results indicate that ZME can be a promising health-functional food material with anti-inflammatory and antioxidant properties.