Immuno-stimulationg polysaccharides from the fruiting bodies of Fomitella fraxinea(I) = Fomitella fraxinea로부터 분리한 면역활성 다당류 (I) : 중성염 용액 추출 다당류의 특성

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Title
Immuno-stimulationg polysaccharides from the fruiting bodies of Fomitella fraxinea(I) = Fomitella fraxinea로부터 분리한 면역활성 다당류 (I) : 중성염 용액 추출 다당류의 특성
Author(s)
Soo Muk Cho; Jae Hoon Lee; Sang Bae Han; Hwan Mook Kim; Seung-Hun Yu; Ick Dong Yoo
Bibliographic Citation
Korean Journal of Mycology, vol. 23, no. 4, pp. 332-339
Publication Year
1995
Abstract
Water-soluble polysaccharide (FCW) was extracted from the fruiting body of Fomitella fraxinea with neutral sodium chloride solution. The polysaccharide was further fractionated into FCW-I and FCW-II by ion exchange chromatography. The FCW-I and FCW-II were then purified by gel permeation chromatography and named as FCW-Ia and FCW-IIa, respectively. FCW-IIa showed relatively strong immuno-stimulating activity but FCW-Ia did not. By analyses of HPLC and GPC, FCW-Ia and FCW-IIa were identified to be homogeneous and their molecular weights were estimated to be about 15,000 and 8,700, respectively. FCW-Ia consisted of fucose, galactose, and mannose as main sugars and their molar ratio was 19.5 : 63.2 : 25.0. Protein was not detected in FCW-Ia. However, FCW-IIa was composed of glucose, galactose, and mannose at a molar ratio of 1.0 : 0.3 : 0.4 and contained 0.4% protein with a higher amount of glutamic acid. A small amount of uronic acid was detected in both FCW-Ia and FCW-IIa.
Keyword
Fomitella fraxineapolysaccharideimmuno-stimulating activity
ISSN
0253-651X
Publisher
Korea Soc-Assoc-Inst
Type
Article
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1. Journal Articles > Journal Articles
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