Fusional expression of human cholesteryl ester transfer protein cDNA in recombinant vaccinia virus-infected mammalian cells

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dc.contributor.authorW H Yoon-
dc.contributor.authorM K Jang-
dc.contributor.authorSong Hae Bok-
dc.contributor.authorY B Park-
dc.date.accessioned2017-04-19T08:44:57Z-
dc.date.available2017-04-19T08:44:57Z-
dc.date.issued1995-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3592-
dc.description.abstractBiologically active human plasma cholesteryl ester transfer protein (CETP) was directed to produce as a fusion protein with glutathione-S-transferase (GST) in recombinant vaccinia virus-infected mammalian cells. Complementary DNAs for GST and CETP were ligated side by side, and introduced into vaccinia viral transfer vectors. The viral vectors were transfected into CV-1 cells infected previously with wild-type vaccinia viruses. Recombinant viruses were generated by the homologous recombination and selected with BUdR and X-gal in human TK~ 143B cells. The DNA from the selected virus was ampliHed and expressed in the CV-1 cells. The GST/CETP fusion protein (86 kDa) was identified on the SDS-PAGE followed by Western blot analysis using polyclonal antibody against the C-terminal active region of CETP fused with GST. The virus-infected cell lysates were loaded on the glutathione-Sepharose affinity column and the CETP was eluted from the column by cleavage with thrombin while the GST/CETP was bound on the column. When subjected to a CETP assay.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleFusional expression of human cholesteryl ester transfer protein cDNA in recombinant vaccinia virus-infected mammalian cells-
dc.title.alternativeFusional expression of human cholesteryl ester transfer protein cDNA in recombinant vaccinia virus-infected mammalian cells-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number2-
dc.citation.endPage113-
dc.citation.startPage107-
dc.citation.volume5-
dc.contributor.affiliatedAuthorSong Hae Bok-
dc.contributor.alternativeName윤우현-
dc.contributor.alternativeName장문규-
dc.contributor.alternativeName복성해-
dc.contributor.alternativeName박용복-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 5, no. 2, pp. 107-113-
dc.description.journalClassY-
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