Efficient expression of human α₁-antitrypsin by the caprine β-lactoglobulin promoter in the mouse mammary cell, HC11

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dc.contributor.authorHyun Ah Kang-
dc.contributor.authorYoung-Ja Song-
dc.contributor.authorEun Joo Seo-
dc.contributor.authorJaeman Kim-
dc.contributor.authorJeong-Sun Seo-
dc.contributor.authorMyeong Hee Yu-
dc.date.accessioned2017-04-19T08:44:57Z-
dc.date.available2017-04-19T08:44:57Z-
dc.date.issued1995-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3596-
dc.description.abstractThe configuration of regulatoiy sequence and protein coding sequence, such as the presence of introns, is considered to be a crucial factor in determining the expression level of the protein of interest. As a preliminary step for generating transgenic animals targeting the expression of human apantitrypsin (apAT) to the mammary gland, we evaluated the feasibility of using the regulatory sequence of the caprine p-lactoglobuline gene to drive expression of the human protein from various vector constructs in a mouse mammary cell line, HC11. The 1.6 kb caprine regulatory sequence supported efficient expression of human ct?AT transcript from the vector constructed with the apAT cDNA sequence. The enhancing effect of arAT intronic sequence on the arAT transcription, however, was not observed in the cells transfected with the vector containing the cti-AT genomic DNA. Both the cDNA construct and the genomic construct showed a similar level of expression for the human ai-AT protein, which was secreted as a glycosylated form into the culture media. The results indicate that intronic sequence of human ai-AT is not absolutely required for the efficient expression driven by the caprine regulatory sequence in the mouse mammary cell.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleEfficient expression of human α₁-antitrypsin by the caprine β-lactoglobulin promoter in the mouse mammary cell, HC11-
dc.title.alternativeEfficient expression of human α₁-antitrypsin by the caprine β-lactoglobulin promoter in the mouse mammary cell, HC11-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number3-
dc.citation.endPage281-
dc.citation.startPage275-
dc.citation.volume5-
dc.contributor.affiliatedAuthorHyun Ah Kang-
dc.contributor.affiliatedAuthorMyeong Hee Yu-
dc.contributor.alternativeName강현아-
dc.contributor.alternativeName송영자-
dc.contributor.alternativeName서은주-
dc.contributor.alternativeName김재만-
dc.contributor.alternativeName서정선-
dc.contributor.alternativeName유명희-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 5, no. 3, pp. 275-281-
dc.description.journalClassY-
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