Cited 1 time in
- Title
- Crystallization and preliminary X-ray crystallographic analysis of DNA polymerase from Thermus aquaticus
- Author(s)
- Soo Hyun Eom; Hyun Kyu Song; Se Won Suh; Young Soo Kim; T A Steitz; Jong Hoon Park; Joong Su Kim; Suk Tae Kwon; Dae Sil Lee
- Bibliographic Citation
- Acta Crystallographica Section D-Biological Crystallography, vol. 32, no. 3, pp. 1086-1088
- Publication Year
- 1995
- Abstract
- Two crystal forms of DNA polymerase from Thermus aquaticus have been grown at room temperature. Rhombohedral crystals (form I) grown from ammonium sulfate solution diffracted poorly to 10 A only and thus are not suitable for X-ray structure determination. Trigonal crystals (form II) grown from polyethylene glycol solution are more suitable for structure determination since their diffraction pattern extends to 2.5 A at cryogenic temperature upon exposure to synchrotron X-rays. They belong to space group P3(1)21 (or its enantiomorph P3(2)21) and their unit-cell dimensions are a = 106.7 and c = 169.7 A, for flash-frozen crystals. The presence of one molecule per asymmetric unit gives a crystal volume per protein mass (V(M)) of 3.0 A(3) Da(-l) and a solvent content of 58% by volume. X-ray data have been collected to 2.7 A Bragg spacing from native crystals.
- Keyword
- DNADNA polymerasecrystallizationthermus aquaticusX ray
- ISSN
- 0907-4449
- Publisher
- Int Union Crystallography
- DOI
- http://dx.doi.org/10.1107/S0907444995003386
- Type
- Article
- Appears in Collections:
- Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
- Files in This Item:
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