Crystallization and preliminary X-ray crystallographic analysis of DNA polymerase from Thermus aquaticus

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Title
Crystallization and preliminary X-ray crystallographic analysis of DNA polymerase from Thermus aquaticus
Author(s)
Soo Hyun Eom; Hyun Kyu Song; Se Won Suh; Young Soo Kim; T A Steitz; Jong Hoon Park; Joong Su Kim; Suk Tae Kwon; Dae Sil Lee
Bibliographic Citation
Acta Crystallographica Section D-Biological Crystallography, vol. 32, no. 3, pp. 1086-1088
Publication Year
1995
Abstract
Two crystal forms of DNA polymerase from Thermus aquaticus have been grown at room temperature. Rhombohedral crystals (form I) grown from ammonium sulfate solution diffracted poorly to 10 A only and thus are not suitable for X-ray structure determination. Trigonal crystals (form II) grown from polyethylene glycol solution are more suitable for structure determination since their diffraction pattern extends to 2.5 A at cryogenic temperature upon exposure to synchrotron X-rays. They belong to space group P3(1)21 (or its enantiomorph P3(2)21) and their unit-cell dimensions are a = 106.7 and c = 169.7 A, for flash-frozen crystals. The presence of one molecule per asymmetric unit gives a crystal volume per protein mass (V(M)) of 3.0 A(3) Da(-l) and a solvent content of 58% by volume. X-ray data have been collected to 2.7 A Bragg spacing from native crystals.
Keyword
DNADNA polymerasecrystallizationthermus aquaticusX ray
ISSN
0907-4449
Publisher
Int Union Crystallography
DOI
http://dx.doi.org/10.1107/S0907444995003386
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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