Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus

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dc.contributor.authorByung Sik Shin-
dc.contributor.authorSeung Hwan Park-
dc.contributor.authorSoo Keun Choi-
dc.contributor.authorBon Tag Koo-
dc.contributor.authorSung-Taik Lee-
dc.contributor.authorJeong Il Kim-
dc.date.accessioned2017-04-19T08:44:59Z-
dc.date.available2017-04-19T08:44:59Z-
dc.date.issued1995-
dc.identifier.issn0099-2240-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3605-
dc.description.abstractDNA dot blot hybridizations with a cryV-specific probe and a cryI- specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific probe hybridized to 12 of the B. thuringiensis strains examined. Most of the cryV-positive strains also hybridized to the cryI-specific probe, indicating that the cryV genes are closely related to cryI genes. Two cryV-type genes, cryV1 and cryV465 were cloned from B. thuringiensis subsp. kurstaki HD-1 and B. thuringiensis subsp. entomocidus BP465, respectively, and their nucleotide sequences were determined. The CryV1 protein was toxic to Plutella xylostella and Bombyx mori, whereas the CryV465 protein was toxic only to Plutella xylostella.-
dc.publisherAmer Soc Microb-
dc.titleDistribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus-
dc.title.alternativeDistribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. en-
dc.typeArticle-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.number6-
dc.citation.endPage2407-
dc.citation.startPage2402-
dc.citation.volume61-
dc.contributor.affiliatedAuthorByung Sik Shin-
dc.contributor.affiliatedAuthorSeung Hwan Park-
dc.contributor.affiliatedAuthorSoo Keun Choi-
dc.contributor.affiliatedAuthorBon Tag Koo-
dc.contributor.affiliatedAuthorJeong Il Kim-
dc.contributor.alternativeName신병식-
dc.contributor.alternativeName박승환-
dc.contributor.alternativeName최수근-
dc.contributor.alternativeName구본탁-
dc.contributor.alternativeName이성택-
dc.contributor.alternativeName김정일-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, vol. 61, no. 6, pp. 2402-2407-
dc.subject.keywordbacillus thuringiensis-
dc.subject.keywordgene location-
dc.subject.keywordbacillus thuringiensis-
dc.subject.keywordbacterial proteins-
dc.subject.keywordgene expression-
dc.subject.localBacillus thuringiensis-
dc.subject.localbacillus thuringiensis-
dc.subject.localgene location-
dc.subject.localBacillus thuringiensis-
dc.subject.localbacillus thuringiensis-
dc.subject.localbacterial proteins-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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