Chemical synthesis and cloning of human β-endorphin gene in Escherichia coli

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Title
Chemical synthesis and cloning of human β-endorphin gene in Escherichia coli
Author(s)
Myung Hee Kim; Hyun Ju Lee; K T Min
Bibliographic Citation
Applied Biochemistry and Biotechnology, vol. 50, no. 1, pp. 35-43
Publication Year
1995
Abstract
Total synthesis of human Β-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the Β-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic Β-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human Β-endorphin gene was cloned into the bacterium Escherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed.
Keyword
Β-endorphinescherichia colihuman geneoligonucleotide synthesisrecombinant DNA
ISSN
0273-2289
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF02788038
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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