Cited 2 time in
- Title
- Chemical synthesis and cloning of human β-endorphin gene in Escherichia coli
- Author(s)
- Myung Hee Kim; Hyun Ju Lee; K T Min
- Bibliographic Citation
- Applied Biochemistry and Biotechnology, vol. 50, no. 1, pp. 35-43
- Publication Year
- 1995
- Abstract
- Total synthesis of human Β-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the Β-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic Β-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human Β-endorphin gene was cloned into the bacterium Escherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed.
- Keyword
- Β-endorphinescherichia colihuman geneoligonucleotide synthesisrecombinant DNA
- ISSN
- 0273-2289
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1007/BF02788038
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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