Overproduction of Serratia marcescens metalloprotease(SMP) from the recombinant Serratia marcescens strains

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Title
Overproduction of Serratia marcescens metalloprotease(SMP) from the recombinant Serratia marcescens strains
Author(s)
K S Kim; K S Park; S M Byun; Jae Gu Pan; Y C Shin
Bibliographic Citation
Biotechnology Letters, vol. 17, no. 5, pp. 497-502
Publication Year
1995
Abstract
To overproduce Serratia marcescens metalloprotease (SMP), various recombinant plasmids encoding SMP gene were constructed and the SMP productivities from the recombinant S. marcescens strains were examined. The recombinant S. marcescens strains indispensably required proteinaceous substrates such as casein for the extracellular production of SMP. We obtained maximum 9,100 U/ml of SMP from the culture supernatant of S. marcescens ATCC27117 containing a regulatory plasmid pTSP2 encoding SMP gene fused with a strong trc99a promoter and its repressor gene lacI(q), which is about 23 times higher than that of wild type strain. SMP produced from the recombinant S. marcescens (pTSP2) was 88.3% of total extracellular proteins.
Keyword
recombinant plasmidserratia marcescens
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00132017
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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