High level expression and simple purification of recombinant human granulocyte colony-stimulating factor in E. coli

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Title
High level expression and simple purification of recombinant human granulocyte colony-stimulating factor in E. coli
Author(s)
Soo-Hyung Kang; Kyu-Heum Na; Jang-Hyeon Park; Choong-Il Park; Se-Yong Lee; Young Ik Lee
Bibliographic Citation
Biotechnology Letters, vol. 17, no. 7, pp. 687-692
Publication Year
1995
Abstract
A human granulocyte colony-stimulating factor (hG-CSF) gene was synthesized and inserted into a trp expression vector for overexpression in E. coli. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabilished. The final productivity of hG-CSF was 500~600 mg per εe culture, and the purified hG-CSF showed the proliferation of neutrophils in vivo assays.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00130351
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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