Cited 11 time in
- Title
- High level expression and simple purification of recombinant human granulocyte colony-stimulating factor in E. coli
- Author(s)
- Soo-Hyung Kang; Kyu-Heum Na; Jang-Hyeon Park; Choong-Il Park; Se-Yong Lee; Young Ik Lee
- Bibliographic Citation
- Biotechnology Letters, vol. 17, no. 7, pp. 687-692
- Publication Year
- 1995
- Abstract
- A human granulocyte colony-stimulating factor (hG-CSF) gene was synthesized and inserted into a trp expression vector for overexpression in E. coli. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabilished. The final productivity of hG-CSF was 500~600 mg per εe culture, and the purified hG-CSF showed the proliferation of neutrophils in vivo assays.
- ISSN
- 0141-5492
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1007/BF00130351
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.